|
Effect of Silymarin on DOCA salt Streptozotocin induced diabetic hypertension in unilateral nephrectomized rats.
|
|
| Author:
|
UPLOADED BY-ADMIN, G. B. JADHAV, C. D. UPASANI
|
| Abstract:
|
The objective of the present investigation was to evaluate the therapeutic efficacy of Silymarin in DOCA salt Streptozotocin
induced diabetic hypertension in unilateral nephrectomized rats.Unilateral nephrectomy was performed in female Wistar
rats (150-200 g). A week after unilateral nephrectomy, diabetic hypertension was produced by administration of single
dose of Streptozotocin (60 mg/kg, i. p.) and Deoxycorticosterone acetate (DOCA) (25mg/kg, once a week; s.c; for 4 weeks)
dispersed in cottonseed oil. The effect of Silymarin (300 mg/kg and 500 mg/kg, p. o., for 4 weeks) was evaluated in DOCA
salt streptozotocin induced diabetic hypertension in unilateral nephrectomized rats. Systolic blood pressure (BP), blood glucose
level and lipid profile was measured once every week during the treatment schedule. After completion of treatment
schedule, heart rate, arterial blood pressure and vascular reactivity to various drugs were recorded. Rats from individual
group were sacrificed, the heart was dissected out and antioxidant enzyme level SOD, CAT, GSH and TBARS were measured.
Urinary Na+and K+ excretions were measured by flame photometer. The isolated heart of rat from each group was also
subjected to histopathology study.Silymarin (300, 500 mg/kg/day, p.o.) significantly (p<0.05) reduced systolic blood pressure,
blood glucose level, heart rate, basal arterial blood pressure and pressor responses to NA, Adr, PE and 5-HT in animals
treated with DOCA salt streptozotocin as compared with diabetic hypertensive rats. Silymarin significantly increased antioxidant
enzyme level of SOD, CAT, GSH, urinary K+ excretion and decreased GSH level, urinary Na+ excretion compared
with diabetic hypertensive group. Silymarin exhibits significant antihypertensive and antidiabetic activity in DOCA salt
Streptozotocin combine model of diabetic hypertension.
|
| Keyword:
|
Hypertension; Diabetes; Silymarin; DOCA; Streptozotocin; Antioxidant.
|
| EOI:
|
-
|
| DOI:
|
-
|
| Download:
|
Request For Article
|
|
|