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In vitro Efficiency of Ampicillin, Thymol and Their Combinations against Virulence Strains of Klebsiella pneumoniae
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| Author:
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SARA MOHAMED, MARY S. KHALIL, MAGDA AZMY
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| Abstract:
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Klebsiella pneumoniae is one of the most common pathogen associated with community and hospital acquired
infections.Widespread antimicrobial resistance often limits the availability of therapeutic options to only a very few
efficacious antibiotics. Therefore, the aim of this study was to test the effect of ampicillin/thymol combination against
planktonic and biofilm cells of ampicillin-resistant Klebsiella pneumoniae.Activity of ampicillin and thymol on planktonic
Klebsiella pneumoniae strains were tested by micro-broth dilution method. Synergistic assay was performed using
checkerboard assay as well asmodified disc diffusionmethods.Ampicillin, thymol and the best synergistic combination
were tested to eradicate the bacterial biofilm by tissue culture plate (TCP) method which were further confirmed by
scanning electron microscope (SEM).High resistance to ampicillin and thymol was detected. The strains affected by
combinations differently, but most of them found to enhance ampicillin activity at concentration 64 µg/ml when
combined with 0.78125 µg/ml of thymol. Biofilm eradication activities of ampicillin (64 µg/ml) and thymol (0.78125
µg/ml) were ranged between 3.258%- 38.879% and 15.452%-55.915% respectively. Ampicillin/thymol combination
eradication activity was ranged from 11.936% to 68.604% which then confirmed by SEM.Thymol combination with
ampicillin was showed to be a strong in vitro antibacterial agent against biofilm formers Klebsiella pneumoniae. In
conclusion more studies and evaluations are in need to validate the use of modified disc diffusion method as a primary
screening technique to detect synergy. In future, further studies on natural compounds are in need which may be an
option to enhance the other commercial antibiotics.
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| Keyword:
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Thymol; Ampicillin; Biofilm; Klebsiella; SEM.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2019.11.03.052
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| Download:
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