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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

A Step Towards Excellence
Published by : Advanced Scientific Research
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0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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Green synthesis of copper nanoparticles (CuNPs) using Melastoma malabathricum leave extract and their antibacterial activity against Escherichia coli and Staphylococcus aureus

Author: IRSHAD HAQ, MUHAMAD NAJIB BIN ABDUL RAHMAN
Abstract: The extensive use of nanoparticles in target specificity and nano-medication had led to variety of methods of synthesis nanoparticles. In this study, plant based green synthesis method was adopted to prepare copper nanoparticle (CuNPs) using Melastoma malabathricum leave extract. The prepared (CuNPs) copper were characterized by UV-visible spectroscopy (UV-vis), X-ray Powder Diffractometery (XRD), X-ray florescence (XRF) and Fourier Transform Infrared Spectroscopy (FT-IR). The UV-visible spectra of CuNPs revealed abroad absorption band between 370 and 650 nm contributing to the transition localized flavonoid system and surface Plasmon vibration of CuPNs, respectively. The XRF analysis confirmed the presence of copper in nanoparticles and XRD result revealed the 13-29 nm size of CuNPs. The presence of (O-H), alkene (C=C), and oxygen ether (C-O) was confirmed by FT-IR analysis. Antibacterial activity of CuNPs against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) was carried out by disc diffusion method with increasing concentration of nanoparticle. The enhanced inhibition zone was observed. The generated nanoparticles exhibited a nano-character with promising bacterial inhibiting activity.
Keyword: Green synthesis, Melastoma malabathricum, XRF, XRD, FTIR, E.Coli, S. aureus
DOI: https://doi.org/10.31838/ijpr/2019.11.03.072
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