|
Stability indicating HPLC method for the quantification of ubidecarenone and piperine simultaneously in tablet formulations
|
|
| Author:
|
KADALI JAGADEESH, NOWDURI ANNAPURNA
|
| Abstract:
|
In this investigation, a stability indicating HPLC method was presented for the simultaneous determination
of ubidecarenone and piperine in bulk and tablets. The ubidecarenone and piperine were analyzed on
Agilent C18 column (250 mm length, 4.6 mm identification, 5 µm particle size) using K2HPO4 buffer (0.1M,
pH – 3.5): acetonitrile (60: 40, v/v) as the mobile phase. The quantity of ubidecarenone and piperine was
quantified by photodiode array detector at 238 nm. The method was validated as per directives given by
ICH. The method showed good linearity with R2 = 0.9992 in the concentration range 2.5-7.5 µg/ml for
piperine and R2 = 0.9991 in the concentration range 100-300 µg/ml for ubidecarenone. The LOD and LOQ
were 0.074 µg/ml and 0.248 µg/ml for piperine, and 0.564 µg/ml and 1.882 µg/ml for ubidecarenone.
Tablet solution of ubidecarenone and piperine was exposed to forced degradation by 0.1 N NaOH
(alkaline hydrolysis), 0.1N HCl (acidic hydrolysis), distilled H2O (neutral hydrolysis), H2O2 (oxidation), dry
heat (thermal degradation) and exposure to sunlight (photo degradation) conditions. The degraded
products are resolved well from the peaks of ubidecarenone and piperine with different retention time.
The validation results proved that the method is selective, precise, robust and accurate for the estimation
of ubidecarenone and piperine simultaneously. The developed stability indicating HPLC method can be
applied for the quantitative determination of ubidecarenone and piperine in bulk drug and tablets.
|
| Keyword:
|
Ubidecarenone, Piperine, Degradation, stability, HPLC, Evaluation
|
| EOI:
|
-
|
| DOI:
|
https://doi.org/10.31838/ijpr/2019.11.03.076
|
| Download:
|
Request For Article
|
|
|