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Lactobacillus paracasei A26 mediated biosynthesis of silver nanoparticles and their antibacterial activity against CAUTIs pathogens
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| Author:
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AL-BASHEERA. H, AL-WANDAWIS
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| Abstract:
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The use of microorganisms for biosynthesis of silver nanoparticles (AgNPs) is considered the eco-friendly technology
and cost-effective routes. The involvement of Lactobacillus spp. for biosynthesis of AgNPs offers a good bio-alternative,
due to most of included members is classified as GRAS, and recently become a research topic in metal nanoparticles
production. In this study, human enteric Lactobacillus paracasei A26 was used for biosynthesis of AgNPs. The nitrate
reductase was mediated the reduction of Ag+ to Ag0. The Nano-scaled silver was achieved at 1mM AgNO3 precursor
concentration, 37°C, and under visible light conditions. The surface Plasmon resonance found at 400 nm, confirmed
the formation of Ag NPs. The particles showed spherical shape with size ranges 5-10 nm by TEM analysis. XRD
analysis confirmed the crystalline nature of the particles. FTIR analysis was demonstrated the biomolecules
responsible in bioreduction of silver ions. Antimicrobial assay verified bactericidal activity of biosynthesized AgNPs
against CAUTIs pathogens, with highest efficacy against Gram- negative uropathogenes. The MIC values were ranged
from 20 to30 µg/mL. Peudomonas.aeruginosa had the lowest MIC with a value of 20 µg /mL. Escherichiacoli, Klebsiella.
pneumonia, Enterobactercloacae, Protease mirabilis and Staphylococcus aureus showed the MIC value of 25 µg/mL,
while Serratiamarcescens, and Streptococcusagalactiae had the highest of all the MICs determined (30 µg/mL).
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| Keyword:
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Metal nanoparticles, silver nanoparticles, L. paracasei, CAUTIs pathogens, antibactericidal activity of AgNPs, and MIC
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2019.11.04.003
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| Download:
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