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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

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Published by : Advanced Scientific Research
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0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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Isolation and Diagnosis of Fungal Isolates Producing Antimicrobial Agents from Petroleum Soil

Author: ALAA HUSSEIN
Abstract: Objective: The present study aims to isolate and diagnose of fungi from soil polluted with oils and investigate their antimicrobial ability. Methods: Soil samples were collected from different areas of oil-polluted soils in Karbala city, Iraq for the period from September to December 2019, cultured on the potato dextrose agar(PDA). A week later, the purification processes were carried out to obtain pure colonies of isolated fungi. The isolated fungi were diagnosed.The fungal isolates were then inoculated in the nutrient broth. After 10 days of incubation, the filtration and centrifuge processes were measured for production media. Then the antimicrobial activity were measured by microtiter plate method. Results:Twenty-four fungal isolates were obtained in this study and are belong to five fungal species, including: Aspergillus niger ( 9 isolates ) , Aspergillus flavus ( 4 isolates ), Aspergillus fumigatus ( 1 isolate ) , Rhizopus stolonifera ( 4 isolates ) and Phoma medicaginis Malbra var. pinodella ( 6 isolates ). Six filtrate fungal isolates out of 24 fungal isolates display an antimicrobial activity versus E. coli, five filtrate fungal isolates display an antimicrobial activity versus Staphylococcus aureus, three filtrate fungal isolates display an antimicrobial activity versus Klebsiella sp and three filtrate fungal isolates showed an antimicrobial activity against Candida albicans. Conclusions:The fungal species found in the soil polluted with oils in the city of Karbala are five fungal species. Some of these isolates have antimicrobial activity versus gram positive and negative bacteria in addition to Candida albicans.
Keyword: Fungi, Soil, Oil, Antimicrobial activity.
DOI: https://doi.org/10.31838/ijpr/2020.12.01.136
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