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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

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Published by : Advanced Scientific Research
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0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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Antioxidant Potential of Aqueous Extract of Entire Plant of Uraria Picta Desv.

Author: UPLOADED BY-ADMIN, BHARATKUMAR D PATEL, YOGESH H KAMARIYA, MANDEV B PATEL
Abstract: The plant Uraria picta desv.(Fam. Papilionaceae) a suffruticose sparingly branched perennial herb. Traditionally, the plant is used as an antidote to the venom of a dangerous Indian snake, leaves are antiseptic and used against gonorrhoea. The fruits, pods are effective against oral sores in children, roots are being used against cough, chills, fever. The main objective of the present study is to access the antioxidant potential of aqueous extract of Uraria picta desv. The different in-vitro models were studied for its free radical scavenging property viz. DPPH radical scavenging assay, ABTS radical scavenging assay, Ophenanthroline assay, lipid peroxidation assay, superoxide scavenging assay, total antioxidant and non-enzymatic haemoglobin glycosylation assay. The results were analyzed statistically by regression method. Its antioxidant activity was estimated by IC50 value and the values were 152 µg/ml (DPPH radical scavenging), 112 µg/ml (ABTS radical scavenging), 29.31 µg/ml (O-phenanthroline assay), 86.39 µg/ml (lipid peroxidation), and 23.87 µg/ml (superoxide scavenging). Total antioxidant capacity of aqueous extract of Uraria picta desv.(10mg/ml) is equivalent to 44.81 mg/ml of ascorbic acid. It showed 74.54% inhibition in non-enzymatic haemoglobin glycosylation assay. In conclusion, significant antioxidant activity was associated with presence of phenolic, flavonoid, sterol and terpene derivatives.
Keyword: DPPH, ABTS, lipid peroxidation, Uraria picta, Papilionaceae.
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