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Evaluation of Mucoadhassive Gel of Liposomal Tetracycline (MAG Lipo T) of Antibacterial Vaginitis
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| Author:
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IMAN AL-SHATI
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| Abstract:
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Introduction: The objective of this experiment was to assess the antibacterial effect and activity of Nano
liposome entrapped tetracycline and compared with ordinary tetracycline against different vaginal isolate
bacterial strains from ewes estrus cycle programmed synchronization by sponges impregnated by Medroxy
progesterone acetate. Methods: Four groups of ewes induced estrous synchronization by vaginal sponges and
examined their sequel vaginal infection by BV blue test obtained the recovery of vaginitis in MAG Lipo T (as
mucoadhesive tablet) higher than other groups and evaluation of antibacterial activity for different tetracycline
formula of Liposome and ordinary tetracycline was carried out using the dilution assay and determination of
minimum inhibitory concentration (MIC) and Minimum bactericidal concentration (MBC) on types of bacterial
strain isolated from vagina. Results: the prevalence of bacterial isolate showed Staphylococcus species
52.3±4.925 and Escherichia coli 43.5±3.781 higher incidence than other strains Klebsiella pneumonia,
Micrococcus species, and others. The MAG Lipo T showed MIC lower than tetracycline against
Staphylococcus spp.0.031±0.004 µg/ml and 2.74±0.035 µg/ml, Escherichia coli 1.632±0.005 µg/ml and
6.52±0.166 and Klebsiella pneumonia 0.012±0.007 µg/ml and 0.51±0.031 µg/ml, Micrococcus spp. 0.164±0.095
µg/ml and 2.29±0.072 µg/ml respectively, the MBC values MAG Lipo T was showed 0.258±0.061 µg/ml,
4.351±0.245 µg/ml, 0.738±0.129 µg/ml and 2.650±0.018 µg/ml against Staphylococcus spp., Escherichia coli,
Klebsiella pneumonia. Micrococcus spp. respectively lesser than ordinary tetracycline 16.25±1.963 µg/ml,
33.71±2.041 µg/ml, 11.58±1.680 µg/ml and 14.34±2.815 µg/ml. in addition, the Bacterial Cell count frequency
distribution (CFU) ml-1 showed lower values in MAG lipo T than other groups. Conclusion: The findings of
this study indicate that Nano liposome entrapped of the mucoadhesive tablet has antibacterial more officious
than ordinary tetracycline against the vaginal bacterial types. This activity supports their practical usage in the
prevention or treatment of vaginal infection due to the application of sponges.
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| Keyword:
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Antibacterial, Tetracycline, Liposome, MIC, Vaginitis, Medroxyprogesterone acetate Sponge, Nano.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.02.0148
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| Download:
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