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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

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Published by : Advanced Scientific Research
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0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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Synthesis, characterization, DNA-interactions, antioxidant, antimicrobial and anticancer studies of mixed-ligand titanocene dichloride complexes

Author: ARCHANA THAKUR, KIRAN NEHRA, SAROJ ARORA, RAJ KAUSHAL
Abstract: The new titanocene dichloride complexes of composition [Cp2Ti(L)(LI-V)] Cl (where HL = 1-adamantylamine, LI-V = Tryptophol, 5-Methoxyindole, Indole-5-Carboxaldehyde, 5-Cyanoindole, 6-Nitroindole respectively) were synthesized and characterized using elemental analysis and different spectroscopic techniques. The synthesized complexes were evaluated for their binding efficacy with calf-thymus DNA (ct-DNA). Complexes (1, 3 and 4) bind to ct-DNA through non-intercalation mode while complexes (2, 5) exhibited intercalation binding mode. Further, complexes were investigated for their antioxidant potential using DPPH assay. Complex 2 exhibited highest antioxidant activity out of all synthesized complexes. Gel electrophoresis technique was employed to investigate the DNA cleavage ability of complexes against DNA plasmid pBR322. Gel electrophoretic results suggested that complexes 3, 4 and 5 had the ability to change the supercoiled form of DNA plasmid to open circular form. Results of antibacterial activity studies using agar well diffusion technique displayed that complex 1 exhibited more activity against E. coli and L. monocytogenes than free ligand. Complexes 2, 3 and 4 had better antibacterial activity than their respective ligands against E. coli. Antifungal activity data stated that complex 3 had more potent activity than its ligand against A. alter strain. In addition, in-vitro anticancer activity of ligands (LI-V) and their respective titanocene complexes was investigated using MTT assay on L6 immortalized cell line and L929 cancerous cell line. Complexes 1, 2 and 4 displayed better cytotoxicity than their ligands against L6 cell line. Complex 2 displayed better cytotoxicity than other complexes against both cell lines (L6 and L929).
Keyword: Titanocene dichloride, 1-adamantylamine, Indole, Gel electrophoresis, DNA plasmid, MTT assay.
DOI: https://doi.org/10.31838/ijpr/2020.12.03.164
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