*Five Years Citation in Google scholar (2016 - 2020) is. 1451*   *    IJPR IS INDEXED IN ELSEVIER EMBASE & EBSCO *       

logo

INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

A Step Towards Excellence
Published by : Advanced Scientific Research
ISSN
0975-2366
Current Issue
Article In Press
No Data found.
ADOBE READER

(Require Adobe Acrobat Reader to open, If you don't have Adobe Acrobat Reader)

Index Page 1
Click here to Download
IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

Click to download
 

Article Detail

Label
Label
Varicella zoster virus based real time PCR identification using a novel nucleic acid extraction method: Samples from Iraq

Author: ANWAR SAIHOOD, AREEJ SALIH SAIHOOD, AHMED RAHEEM RAYSHAN
Abstract: Varicella zoster virus (VZV) can cause varicella (chickenpox) infection that can be serious in people, infants and adults, who are immunecompromised. The rapid detection of the infection is important especially in the immune-weakened patients for better control of the virus. This requires finding new-shortcut-procedures that reduce the time spent for sample processing in the laboratory. Here, the current survey was intended to diagnose VZV from patients, children, Al-Diwaniyah province, Iraq. Samples, 65 patients, were collected from open blister lesions using sterile swabs. The samples were subjected to two initiating processes, a commercial kit (CK) or homogenization-heat-C3, a commercial patented solution, (HHC3) ahead of performing of a real-time PCR (RT-PCR) method. The outcomes revealed significant (p?0.05) increases, as 60(92.3%) of identification, in the detection of the VZV using HHC3 methods after performing the RT-PCR especially when compared with the CK, as 52(80%) method. The sensitivity was significantly higher (p?0.05), 90%, from HHC3 than that from CK, 85%. The current investigation ensures a faster processing procedure, HHC3, for detecting the Varicella zoster virus from clinical specimens than the usual methods used for nucleic acid extraction.
Keyword: Nucleic acid extraction, RT-PCR, Varicella zoster virus.
DOI: https://doi.org/10.31838/ijpr/2020.12.03.187
Download: Request For Article
 




ONLINE SUBMISSION
USER LOGIN


Username
Password
Login | Register
News & Events
SCImago Journal & Country Rank

Terms and Conditions
Disclaimer
Refund Policy
Instrucations for Subscribers
Privacy Policy

Copyrights Form

0.12
2018CiteScore
 
8th percentile
Powered by  Scopus
Google Scholar

hit counters free