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Extractive Spectrophotometric Methods for the Determination of Deflazacort in Pharmaceutical Dosage Forms
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| Author:
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UPLOADED BY-ADMIN, SATISH A. PATEL
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| Abstract:
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Two simple, rapid, accurate, precise and economic spectrophotometric methods have been developed for the estimation of
deflazacort in pharmaceutical formulation. These methods are based on the formation of chloroform soluble ion-association
complexes of deflazacort with methyl orange in a phosphate buffer of pH 4.0 (Method I) and with bromothymol blue in a
phosphate buffer of pH 6.8 (Method II). This property of the drug was followed for the development of colorimetric methods
for analysis of drug. The complex of deflazacort with methyl orange and bromothymol blue showed ?max at 425 nm and 415
nm, respectively. The absorbance was found to increase linearly with increasing concentration of deflazacort, which was
corroborated by the calculated correlation coefficient values (0.9970 and 0.9956, respectively for method I and method II).
The systems obeyed the beer law in the range of 3-20 and 5-24 µg/ml for methyl orange and bromothymol blue,
respectively. Recovery studies gave satisfactory results indicating that none of common additives and excipients interfere the
assay method. Various analytical parameters were evaluated and the results were validated by statistical data. The proposed
methods are found to be simple, sensitive, accurate, precise and reproducible hence can be successfully applied for the
analysis of deflazacort in pharmaceutical dosage form.
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| Keyword:
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Deflazacort, Methyl Orange (MO), Bromothymol Blue (BTB), Colorimetric analysis, Extractive spectrophotometry.
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| DOI:
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