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Detection of lmrS, mepA, norC genes among Staphylococcus Aureus Isolates
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| Author:
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MARWA JEAD, RANA KADHIM MOHAMMED
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| Abstract:
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Staphylococcus aureus is bacterium present in skin and upper respiratory tract. Although S.aureus is normal flora also become opportunistic pathogen cause many infection that difficulty treatment because of presence virulence gene. The purpose of this study is to screen the antibiotic susceptibility patterns and the prevalence of lmrS, mepA, nor Cgenes among bacterial isolates. In the present study, 213 samples were collected from different clinical sources from patients who admitted different hospitals in Baghdad city in period ranged 23/11/2018-18/3/2019. The isolates were diagnosed as S.aureus based on routine bacteriological methods and confirmed by molecular method using 16s rRNA gene. The antibiotic susceptibility test was performed to all identified isolates by Kirby-Bauer Disk Diffusion method using sixteen types of antibiotics. The results of antibiotics susceptibility test revealed high level of resistance toward Azithromycin80%, Erythromycin and Cefoxitin82%, meropenem and ceftriaxone 84% cefotaxime and colistin 100%, methicillin 76%, mecillinam 87% respectively. Finally, the prevalence of lmrS gene among bacterial isolates was detected as 98%, norC 100% and mepA 95%.
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| Keyword:
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Staphylococcus Aureus, lmrS Gene, Antibiotics.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.01.243
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| Download:
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