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Matricaria chamomilla extract inhibits the metastatic potential of colorectal cancer cell line via reduction of matrix metalloproteinase enzymes activity
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| Author:
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HASSAN ABIDI, NAZANIN DANAEI, REZA MAHMOUDI, AREZOO SABET, MANSORE ESMAEILI, MOHSEN NIKSERESHT
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| Abstract:
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Background and objective: Chamomile (Matricaria chamomilla) is a medicinal herb containing antioxidant compounds whose effects have been observed in treatment of several diseases. In present study the anti-angiogenic effect of hydro-alcoholic extract of chamomile has been studied in human colorectal cancer HT-29 cell line.
Method and material: HT-29 cells were cultured in RPMI1640 media. The cells were exposed to different concentrations of different concentrations of hydro-alcoholic extract of chamomile, bevacizumab and both agents combined. Viability was evaluated by (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a tetrazole) assay (MTT). Vascular endothelial growth factor (VEGF) gene expression and the activity of matrix metalloproteinase-2 (MMP-2) and MMP-9 enzymes were assessed in the presence of this extract. Then data were analyzed using SPSS 21 software, through descriptive and inferential statistics at a 95% confidence level. Also, for drawing the graphs we used GraphPad Prism Version 7. In this study, ethical considerations were respected.
Results: The expression of VEGF gene and secretion of VEGF significantly down-regulated in the treated cells group compared to control group (p<0.05). Secretion of VEGF was significantly reduced in cells treated with bevacizumab and the extract synergistically (p<0.05). The activity of MMP-2 and MMP-9 enzymes decreased in the treated cells (p<0.05).
Conclusion: Hydro-alcoholic extract of chamomile can affect metastatic potential of colorectal cells via affecting on MMPs activity and VEGF gene expression.
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| Keyword:
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Chamomile, Angiogenesis Inhibitors, Bevacizumab, MMP-2, MMP-9, Colorectal Neoplasms, HT29 Cells
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.04.002
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| Download:
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