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Trigonella foenum graecum mitigates proteases and restores antioxidant balance in collagen induced arthritis in rats
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| Author:
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UPLOADED BY-ADMIN, M. ASIF, SADIQ UMAR, HAIDER A. KHAN
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| Abstract:
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Autoimmunity driven immune cell infiltration and associated proteolytic enzyme stimulation with concomitant oxidative
stress is responsible for the tissue degradation in human rheumatoid arthritis. In the present study, we investigated the antiinflammatory
and antioxidant potential of hydro alcoholic extract of Trigonella foenumgraecum in collagen induced arthritis
in Wistar rats. Rats were treated with Trigonella foenumgraecum at a dose of 120 mg kg-1 b.wt orally for 21 days and effects
were assessed by biochemical (elastase, LPO, GSH, SOD, catalase and NO) and histological evaluation in joints.The disease
was evident after 13 ± 1 days post induction with increase in paw volume, digital inflammation which progressed to swelling
of multiple joints. A single injection of CIA led significant elevation of neutrophil elastase (p<0.01). Peroxidation of lipids
increased with parallel decrease in reduced glutathione content (p<0.001). Enzymatic antioxidants viz. superoxide dismutase
and catalase showed a similar decrease in the CIA immunized rats. Moreover, nitrite content was seen to be significantly
increased (p<0.001) in the arthritic rats. Trigonella foenumgraecum extract was able to decrease neutrophil elastase
(p<0.001) and decrease free radical overload both of oxygen and nitrogen free radicals to a significant extent. All these
biochemical changes were reflected in the histology which revealed the decrease in infiltration of inflammatory cells after
treatment with Trigonella foenumgraecum against collagen induced arthritis. Our investigation supports the antioxidant and
anti-inflammatory properties of Trigonella foenumgraecum in collagen induced arthritis model and at the same time
highlight neutrophil elastase and oxidative stress to be the targets of therapeutic intervention.
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| Keyword:
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Trigonella Foenumgraecum, articular elastase, nitric oxide, inflammation, Oxidative stress.
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| DOI:
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