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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

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Published by : Advanced Scientific Research
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0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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An In Vitro Evaluation of the Ability of Ozone Nano-bubble Water to Kill Pathogenic Gram-Negative and Gram-Positive Bacteria

Author: AHMED L.OSMAN, REEM ALHATTI, HIBA SAWAS, ABD ELGADIR A.ALTOUM, DEVAPRIYA FINNEY SHADROCH, WALID EL-SAYED, PRAVEEN KUMAR KANDAKURTI, ASAAD MA. BABKER
Abstract: Aim To evaluate the bactericidal effects of ozone Nano-bubble water (NBW3) in different pathogenic bacteria. Methodology 0.1 mL volume of bacteria suspension containing approximately 1 × 107 CFUs/ mL (McFarland standard) of each test bacterium added to each 10 mL of serial dilution of ozone nanobubbles water, 0.9% NaCl with standard strains of bacteria (as negative control) used. Following the incubation (for 2, 5, and 10 min, at 37 °C). The 0.9% NaCl control bacterial suspensions incubated and treated in the same manner. Aliquots removed from each tube at each time point and serial 10-fold dilutions prepared in sterile physiological saline. Next, 0.1 mL aliquots of each dilution aseptically inoculated on plate of agar medium and incubated at 37 °C for overnight. Colonies then be counted, and the number of CFUs/mL be plotted against time for each bacterium tested. Results Ozone had an antibacterial effect on both gram positive and gram-negative bacteria those suspended in first 2 delusion, but little effect in third one. Its antibacterial efficacy was not comparable with that of NaCl under the test conditions used. Ozone-Nano effect on gram negative and gram-positive bacteria. Conclusion Ozone-Nano-bubbles water has bactericidal effect on different strains of gram negative and gram-positive bacteria.
Keyword: Ozone nano-bubble water, Antibacterial, Gram Negative, Gram-positive Bacteria
DOI: https://doi.org/10.31838/ijpr/2020.12.03.367
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