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Activity of a novel Selena-diazole derivative compound on primary neuron cells isolation from cortex and hippocampus of 18th day aged fetus of pregnant Wister rat
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| Author:
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NADHEERAH F.NEAMAH, SHAKER.A.N.AL-JADAAN
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| Abstract:
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Seleno-cysteine amino acid is originated in the active site of all selenium containing enzymes such as glutathione peroxidase. Several selenoproteins in the brain, but their roles were remained not well documented. The aim of the study is to investigate the effect of a novel selena-diazole derivative i.e. 4¯, 4¯¯ - (4, 5, 6, 7- Tetrahydro- [1, 2, 3-] selenadiazolo [4, 5e] pyridine - 4, 6 - diyl) bis (benzene-1, 3-diol) (SeD) on viability of cortical neurons and primary hippocampus cells isolated from prenatal rat pulps at 18th days. Fetal brains were removed and placed in an ice chilled Hibernated E. Hippocampi and cortices were separated. Papain 0.05% added for 10 min at 37º C; then trituration by fire polished-glass pipettes were done. Culture dishes with diameter 60 mm or 24-well plates were coated by Poly D Lysine on a density 5*104/dish. Neurons were cultured on neuro-basal medium complemented with B27 and incubated in a humidified Co2 5% atmosphere incubator at 37º C. SeD in different concentration were added, viability percentage were estimated at zero time, after 1hr, 2hr, 3hr. The results illustrated that a significant decreased in viability in a dose dependent and a time dependent manner in comparison with control and solvent groups. Despite the statistically significant level of cell viability, the levels remain high compared to other selenidiazole derivatives, also it showed the most reduction percentage after incubation for 3 hour of all treated groups, also, high significant reduction observed in 400 and 500 µg/ml treated groups. It can be concluded that SeD compound has a dose and time dependent reduce in cell viability; but still more preserve effects on neuron cells than control groups.
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| Keyword:
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Selenadiazole, primary neuron cells, viability, trypan blue, Acridine Orang Stain.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.04.346
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| Download:
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