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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

A Step Towards Excellence
Published by : Advanced Scientific Research
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0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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Study The Antibacterial Activity Of Uwi Sap (Dioscorea Alata.L) Extracts From Indonesia In Damaging Escherichia Coli And Staphylococcus Aureus Cells.

Author: , A.NURFITRIANI, META MAHENDRADATTA, AMRAN LAGA, ZAINAL
Abstract: Some antibiotics are no longer effective in the treatment of infection because of resistance to microorganisms. The search for new, more effective antimicrobials from plants becomes necessary to continue to do so, especially those derived from natural ingredients. This study aims to determine the potential activity of the uwi plant (Dioscorea alata L) as an antibacterial agent for Staphylococcus aureus and Escherichia coli to its maturity. Testing to see the antibacterial inhibition was carried out using the agar diffusion method. The antibacterial mechanism was carried out using Scanning electron microscopy (SEM). The test results showed that the uwi sap extract had antibacterial activity against Staphylococcus aureus and Escherichia coli, which was characterized by an inhibition zone. The S. aureus bacteria gave the highest inhibition zone at a concentration of 96% with a diameter of the inhibition zone of 11.8 mm, while for E. coli, bacteria gave the highest inhibition zone at a concentration of 50%, namely 11.4 mm. These results also showed that the uwi sap extract could damage morphology and cause leakage of S. aureus and E. coli bacterial cells. So it can be concluded that uwi sap extract has antibacterial activity because it can inhibit the growth of S. aureus and E. coli bacteria.
Keyword: uwi sap extract, antibacterial, S. aureus, E. coli, SEM
DOI: https://doi.org/10.31838/ijpr/2021.13.01.129
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