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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

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Published by : Advanced Scientific Research
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0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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Classification and prediction of patin fish oil’s antioxidant activities from different origins using FTIR spectroscopy and chemometrics

Author: ANGGITA ROSIANA PUTRI, ABDUL ROHMAN, SUGENG RIYANTO, WIDIASTUTI SETYANINGSIH
Abstract: Patin fish oils (PFOs) from fifteen different origins was evaluated the antioxidant activity using 2,2’-diphenyl-1-picrylhydrazil (DPPH) radical scavenging. All PFO samples were analyzed using Fourier Transform Infrared (FTIR) spectroscopy. The spectra were recorded in absorbance mode and processed statistically using multivariate chemometric techniques, including Principal Component Analysis (PCA), Principal Component Regression (PCR) and Partial Least Squares (PLS). PCA was used to classify PFOs from different origins, while PLS and PCR was used to predict the antiradical activity of PFOs based on FTIR spectra. The result showed that radical scavenging activity (%RSA) of PFO from Demak and Boyolali (Central Java) had high value than others, 40.88 ± 0.18% and 40.71 ± 0.07%, respectively. PCA could successfully classify PFOs from fifteen different origins based on the absorbance of FTIR spectra. PCR using the first derivative spectra at 3005-650 cm-1 could predict %RSA of PFOs with R2 value of 0.9987 and 0.9982 in calibration and validation models, with RMSEC and RMSEP were 0.609 and 0.734, respectively.
Keyword: patin fish oils, antiradical scavenging activities, chemometrics, FTIR spectra, geographical origins
DOI: https://doi.org/10.31838/ijpr/2021.13.01.150
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