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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

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Published by : Advanced Scientific Research
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0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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Identification of Phenolic Compound from Active Subfraction Of Baccaurea Racemosa Pulp by High Resolution Mass Spectrometry

Author: LINA PERMATASARI, SUGENG RIYANTO, ABDUL ROHMAN
Abstract: Ethyl acetate fraction from Baccaurea racemosa pulps that have the highest total phenolic content are potentially developed to be natural antioxidant. However, phenolic compounds in ethyl acetate fraction of B. racemosa pulp have not been published. The aim of this study was to determine the antioxidant activity in active subfraction from ethyl acetate fraction of Baccaurea racemosa pulp and identification its phenolic compounds. The ethyl acetate fraction of Baccaurea racemosa pulp was fractionation by gravity column chromatography. All subfraction was determined their antioxidant activity by 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay. The higher antioxidant from subfraction was identified its metabolites by high resolution mass spectrometry (HRMS). In this study, subfraction C was an active subfraction that have the highest DPPH radical scavenging activity with IC50 values 40.134±1.105 µg/mL. An active sufraction have 18 metabolites which classified in phenolic compounds. Gallic acid is the abundant phenolic compound in active subfraction from B. racemosa pulp. We concluded that the phenolic compounds were the one of metabolite in active subfraction of B. racemosa pulps which could play the important role to determine the quality of their antioxidant activity.
Keyword: High resolution mass spectrometry, DPPH radical scavenging activity, metabolite profiling, phenolic compound, Baccaurea racemosa
DOI: https://doi.org/10.31838/ijpr/2021.13.01.171
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