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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

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Published by : Advanced Scientific Research
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0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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Free radical scavenging activity of methanol extract and compounds isolated from stems of Etlingera rubroloba A.D Poulsen

Author: ASRIULLAH JABBAR, SUBAGUS WAHYUONO, I SAHIDIN, IKA PUSPITASARI
Abstract: Etlingera rubroloba (Blume) A.D Poulsen is an endemic plant found in Southeast Sulawesi, Indonesia. So far, there is no report on medicinal usages of this species, therefore expolative study of this species is undertaken. E. rubroloba was subjected to scavenging DPPH free radicals activity method, and then the anti radical active compounds were isolated and identified. Initially this species was extracted, partitioned to give fractions having simple tlc picture, and the compounds were isolated by radial chromatography. The compounds obtained were identified according their spectroscopic (UV, IR, MS, NMR) properties. Two compounds (1 and 2) were isolated and based on spectroscopic data and comparison with reported data, compound 1 was identified as synapyl alcohol diacetate and compound 2 was identified as stigmasterol. The presence of these two compounds were first reported in E. rubroloba. The IC50 (µg/mL) values of 1 and 2 were 182.799, 402.397, methanol extract 41.592 and 20.895 µg/mL was for vitamin C as positive control.
Keyword: Etlingera rubroloba, methanol extract, synapyl alcohol diacetate, stigmasterol, DPPH
DOI: https://doi.org/10.31838/ijpr/2021.13.01.179
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