*Five Years Citation in Google scholar (2016 - 2020) is. 1451*   *    IJPR IS INDEXED IN ELSEVIER EMBASE & EBSCO *       

logo

INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

A Step Towards Excellence
Published by : Advanced Scientific Research
ISSN
0975-2366
Current Issue
No Data found.
Article In Press
No Data found.
ADOBE READER

(Require Adobe Acrobat Reader to open, If you don't have Adobe Acrobat Reader)

Index Page 1
Click here to Download
IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

Click to download
 

Article Detail

Label
Label
Histopathology And Genetics Study Of Sperm Abnormalities In Mice Infected With Brucella Meletensis

Author: EMAN HASHIM YOUSIF, MUNA SACHIT HASHIM, THIKRA ABDULLAH MAHMOOD
Abstract: Background: Mammalian spermatogenesis is a process that includes the propagation and differentiation in seminiferous tubules of the testis cell. It’s characterized by meiotic, mitotic divisions and spermatogenesis. Exogenous and endogenous chemical, physical, and biological causes modify the genome of spermatozoa. Marinating of the genomic stability and integrity of the sperm is a vital event and it is regulated by DNA repair processes; DNA repair processes in male germline involving base excision repair, nucleotide excision repair, DNA mismatch repair, double-strand break repair and post-replication repair. It defects in repair processes cause the arrest of spermatogenesis and abnormal recombination, eventually causing male infertility. Methodology: In this study, about 25 mice were experimentally infected with Brucella melitensis to investigate the pathological alterations on testicular tissues, as well as for detection of DNA damage of daily repairing gene 22q11.2 in Spermatocytes using the FISH technique. Results: samples showed frequently of DNA damage appear as deletion at 22q11.2 gene of spermatocytes. Histopathological examination of mice infected with Brucella melitensis revealed necrotic orchitis with obvious loss of spermatogenesis within seminiferous tubules associated with a severe inflammatory response. Conclusion: genetic regulation of DNA repair process in spermatocytes was losing in brucellosis owing to damage in chromosomal material at gene locus responsible for DNA regular repairing process of the sperm, as well as testicular tissues damage.
Keyword: Brucellosis, DNA damage, FISH technique, Spermatogenesis.
DOI: https://doi.org/10.31838/ijpr/2020.12.03.487
Download: Request For Article
 
Clients

Clients

Clients

Clients

Clients
ONLINE SUBMISSION
USER LOGIN
Username
Password
Login | Register
News & Events
SCImago Journal & Country Rank

Terms and Conditions
Disclaimer
Refund Policy
Instrucations for Subscribers
Privacy Policy

Copyrights Form

0.12
2018CiteScore
 
8th percentile
Powered by  Scopus
Google Scholar

hit counters free