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Enhanced Antifungal Activity of Voriconazole-Loaded Solid Lipid Nanoparticles and Nanostructured Lipid Carriers Against Aspergillus Flavus Isolates from Goat
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| Author:
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ALWAN A. H. AL QUSHAWI, KHAIRI JAMEEL AL-RUABY
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| Abstract:
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Voriconazole is an antifungal agent that is used for treatment of Aspergillus infections, several side effects are connected with it. For overcome the limitations, excellent carriers for antifungal drugs may be nanostructured structures. This study aimed to isolating Aspergillus flavus from goat tracheal swabs also investigates the development of voriconazole-loaded solid lipid nanoparticles and nanostructured lipid carriers, then analyzes their characteristics, and compares the antifungal activities of these preparations with free voriconazole. Aspergillus flavus molds were isolated and defined. Voriconazole-lipid nanoparticles formulations were prepared and physiochemical properties, encapsulation efficiency, and drug release were investigated. Physical stability was characterized and identified for their suspension and dry powder after lyophilization with mannitol as a cryoprotectant and without of cryoprotectant. In vitro antifungal activity of voriconazole-lipid nanoparticles was tested. The lowest particle size values (121.8±5.3) were seen in voriconazole-nanostructured lipid carriers (suspension). Formulations showed zeta potential values ranged from -10.4±0.14mV to -29.3±1.31mV. Polydispersity index values were under 0.3. Morphologically, particles showed smooth surfaces, almost spheroid-like. Maximum values of encapsulation efficiency and drug release with voriconazole-nanostructured lipid carriers (suspension) were recorded. After 90 days, all lyophilized formulations exhibited good water stability. The least initial burst release amongst all experimented formulations was performed by voriconazole-nanostructured lipid carriers (suspension)+mannitol powder at pH 7.4. Voriconazole-nanostructured lipid carriers preparations showed higher antifungal activity against the examined isolates at 0.5µg/mL followed by voriconazole-solid lipid nanoparticles preparations, while free voriconazole resulted in the lowest inhibition values. All formulations, especially voriconazole-nanostructured lipid carriers+mannitol, could represent a promising formula for antifungal application.
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| Keyword:
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Voriconazole, Lipid, Nanoparticles, Nanostructured, Aspergillus Flavus
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.01.407
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| Download:
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