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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

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Published by : Advanced Scientific Research
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0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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Effect of Green Tea (Camellia Sinensis) Extract Administration on Sperm Quality of Mice (Mus Musculus) Exposed To 2,3,7,8-Tetrachlorodibenzo-P-Dioxin

Author: , MEY VANDA PUSPARINA SAJIDA, EPY MUHAMMAD LUQMAN, INDAH NORMA TRIANA, WIDJIATI WIDJIATI
Abstract: The aim of this research is to analyze the effect of green tea extract on motility, viability, and sperm concentration of mice exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). The study used 25 male mice of BALB/c strain with an average weight of 20 gram were used in this research. TCDD was given single dose 7 µg/Kg BW via intraperitoneal injection while green tea extract was given orally for 53 days at a dose as follows: 1, 2, and 4 mg/Kg BW/day. Mice semen were collected then its motility, viability, and concentration were checked. Sperm quality were highest in mice exposed to TCDD and given green tea extract at a dose 4 mg/Kg BW/day than those mice exposed to TCDD and given green tea extract at the lower dose. The sperm motility, viability, and concentration mean were sequentially: 72.57 ± 5.20%;76.48 ± 5.61%; 6.97 ± 0.11 million/mm3. In conclusion, administration of green tea extract at a dose of 4mg/Kg BW/day could maintain sperm motility, viability and concentration from a damage caused by TCDD.
Keyword: green tea extract, motility, viability, concentration, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)
DOI: https://doi.org/10.31838/ijpr/2020.12.04.319
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