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Molecular typing using RAPD – PCR of Multidrug resistance Escherichia coli isolated from patients in Diyala province / Iraq
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| Author:
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SABA J.JAWAD AL – ZUBAIDI
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| Abstract:
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Urinary tract infections (UTI) are extremely popular infections that described by bacterial colonization of the urinary system. UropathogenicEscherichia coli is commonly pathogens isolated from UTI patients. The current study included collection of sixty seven samples related to urinary tract infection patients in Baquba Teaching Hospital and Al - Batoul Teaching Hospital in Diyala, during the period from 28 September to (28) November 2019. All isolates were identification as E.coli by using Gram – staining, EMB agar, biochemical tests and confirmed by VITEK2 compact system. It was found (23.88%; n = 16)E.coli isolates were Multidrug resistance. The MDR E.coli were showed high resistance against (CPO 93.75%, ATM 87.5%, STX 81.25%,Dox and CFX 75%, TE and AZM 67.75%, LVX and TCC 50%, FOX 31.25%, IMI 25%, AMS 0%, the Phenotype study of Biofilm formation using microtiter plate method, show (87.5% ; n = 14) isolates were moderate biofilm and (12.5% ; n = 2) isolates were weak biofilm. The results of genotyping using the RAPD – PCR technique were within two main groups, group A and group B, So the largest percentage of group B was for clinical isolates and by 80% (8 clinical isolates) within two subgroups are B1 and B2 and by 10% (one clinical isolate) of the group A clinical isolates, one the clinical out group A and group B isolates,it indicates that a correlation between E.coli isolates when using RAPD – PCR typing.
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| Keyword:
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Multidrug resistances,Escherichia coli, urinary tract infection, RAPD – PCR.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.02.083
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| Download:
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