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A Colorimetric Analysis Study of Uric Acid in Goat Patients
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| Author:
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ABBAS SHEBEEB HASAN AL-KADUMI, NOOR MAZIN IBRAHIM, DUHA BAHAA MOHAMMED AL-FAYAAD
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| Abstract:
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Simple, sensitive, precise and accurate colorimetric method for the analysis of uric acid was proposed. The
method was based on the complexing reaction between uric acid with potassium hexacyano ferrate and ferric
chloride in a hydrochloric acid medium to form Prussian blue color complex, the optimum conditions were
(maximum wavelength was 735 nm, volume 0.3 ml for hydrochloric acid,1.25 ml for both Potassium hexacyano
ferrate and ferric chloride solutions, 60 ºC and 8 minutes as maximum temperature and time for the reaction,
the order of mixing additives was uric acid, ferric chloride, Potassium hexacyano ferrate and hydrochloric
acid). The highly accurate and precise results obtained with RSD%, Recovery%, Ere% and D.L were (0.755 to
4.376), (99.676 to 97.831), (-0.033 to -2.169), and 0.1569 mg.L-1, respectively. The linearity was in the range
between (2.0-200 mg.L-1). The proposed methods were successfully applied for the analysis of uric acid in
serum and urine samples for twenty persons
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| Keyword:
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Uric acid, colorimetry, analysis, gout patients
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| EOI:
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-
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.02.153
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| Download:
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