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Fasting Techniques – A Novel Approach for Cancer Therapy with Cyclophosphamide Loaded Nanoparticles on DAL-Mice
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| Author:
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TANUDEEP DATTA, DHRUBJYOTI MUKHERJEE, MOHAMMAD AZAMTHULLA
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| Abstract:
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Objective: To evaluate the effect of Nanoparticles loaded with cyclophosphamide by implementing various
fasting techniques for anticancer activity in DAL mice.
Methods: Nanoparticles were formulation using the technique of Ionic Gelation. Sodium alginate
cyclophosphamide nanoparticles were synthesized and characterized with SEM, AFM and Tyndall effect. Two
fasting models namely, intermittent fasting and alternate day fasting technique was used in the present study.
Mice were divided into 10 groups under each model. All the groups were inoculated with DAL cell line, except
normal group and this was considered as Day ‘0’. 24hrs after inoculation all the groups were treated as per the
following protocol. At the end of study period all the, hematological parameters, body weight measurements,
tumour volume, life span and survival time measurement were analyzed.
Results: The drug loaded nanoparticles was developed and particle size are ranging from 59 to 149nm.
Intermittent fasting and alternate day fasting techniques showed significant anticancer activity when compare to
positive control. The animals treated with nanoparticle loaded cyclophosphamide with fasting techniques
showed highly significant anticancer activity then standard cyclophosphamide alone treated animals.
Conclusion – Fasting approaches can bring significant changes in the current cancer therapy. Further, more
detailed studied could be conducted to calibrate the fasting treatment approach in a graded manner. A
combination of anticancer drug and fasting can lead to immense benefits, effectivity and a huge drop in side
effects.
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| Keyword:
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Cancer, Nanoparticles, Lymphoma, Fasting, Mice.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.02.278
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| Download:
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