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Evaluation of Antioxidant, Antibacterial and Cytotoxic Activity of a Polyherbal Formulation, Slimkar 19b
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| Author:
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PRABHUPRASAD PADUCHURI, DR PHILOMENA GEORGE
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| Abstract:
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Aim: The aim of the present study was to evaluate the antioxidant, and antibacterial activity of a polyherbal formulation, Slimkar 19b. Study was also undertaken to assess the cytotoxicity in 3T3L1 cell lines.
Methodology: We amply determined the antioxidant activity of the slimkar19b with standard assays viz, 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), Ferric Reducing Antioxidant Power (FRAP) and 1,1-diphenyl-2-picrylhydrazyl (DPPH). Antibacterial activity of slimkar19b was assessed against perilous pathogens by resazurin assay. The cytotoxicity of formulation was checked by MTT assay against 3T3L1 cell lines.
Results: The slimkar19b IC50 value of DPPH, ABTS and FRAP assay was found to be 393.64±10.2µg/mL, 195.77±7.34µg/mL and 82.97±4.2mg/mL respectively. The minimum inhibitory concentration (MIC) values against test organisms Bacillus cereus, Staphylococcus aureus, Streptococcus mutans, Escherichia coli, Pseudomonas aeruginosa and Salmonella typhi were found to be 500µg/mL, 125µg/mL, 500µg/mL, 1000µg/mL, 1000µg/mL and 1000µg/mL respectively. The revealed data showed that Slimkar19b was toxic to 3T3L1 Cell Lines at 649.76±24.20 µg/mL (IC50) concentration.
Interpretation: The study has shown the antioxidant and antibacterial potential of Slimkar19b, a polyherbal formulation.
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| Keyword:
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Slimkar19b, Polyherbal Formulation, Antioxidant, Antibacterial activity, Cytotoxicity, etc.
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| EOI:
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-
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.02.421
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| Download:
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