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In vitro antioxidant and antibiofilm activities of Microcystis sp. against multidrug-resistant human pathogens
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| Author:
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R.KANIMOZHI , D.ARVIND PRASATH, R.DHANDAPANI, SANTHOSH SIGAMANI
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| Abstract:
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The biofilm of drug-resistant pathogenic bacteria is a serious issue worldwide. The natural growth of blue-green algae Microcystis sp. has the potential to antagonize the bacterial pathogens in a novel approach. In the present study, Microcystis sp. was isolated and its mass production was optimized by Response Surface Methodology (RSM) and also, the in vitro antioxidant and antibiofilm potentials against the drug-resistant pathogens were investigated. The experimental design was carried out on mass production of Microcystis sp. with pH, and mineral salts as independent variables. The maximum biomass yield (1.5 g/L) was achieved with NaNO3 (20mM) and K2HPO4 (10mM) at pH 7.5. The Microcystis sp. exhibited remarkable antioxidant (65%) and antibiofilm activities against Staphylococcus aureus and Pseudomonas aeruginosa. The Gas chromatography-mass spectrometry (GC-MS) of Microcystis sp. extract analysis revealed the presence of sixteen bioactive potential compounds which proved the synergistic activity against the bacterial pathogens. Therefore, the potential bioactive compounds in Microcystis sp. could be an effective biocontrol agent against the challenging drug-resistant bacterial pathogens.
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| Keyword:
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Antioxidant activity; Biofilm; Cyanobacteria; Microcystis sp., Response surface methodology
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.02.476
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| Download:
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