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Reversed Phase High Performance Liquid Chromatographic (Rp-Hplc) Method For Estimation Of Berberine Hydrocholide In Bulk And Pharmaceutical Nanoparticles
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| Author:
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SNEHAL PATEL, CHINTAN AUNDHIA, KARTIK PANDYA
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| Abstract:
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An accurate, simple, and reproducible liquid chromatographic method was developed and validated for the determination of Berberine in nanoparticles. The analysis was performed at ambient temperature on a reversed-phase C18 column with UV detection at 289 nm. The mobile phase was freshly prepared 50mM potassium dihydrogen phosphate buffer (pH 3.0 adjusted using orthophosphoric acid): acetonitrile in a ratio of 70:30 v/v used at a constant flow rate of 1 ml/min. The method was validated in terms of linearity, precision, accuracy, and specificity. The response was linear in the range of 10-50 µg/ml (r2 = 0.9964). The relative standard deviations for intra- and inter-day precision studies were found to be less than 2% which indicates method is precise.
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| Keyword:
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Berberine hydrochloride, RP-HPLC, Validation, ICH.
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| EOI:
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-
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.03.006
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| Download:
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Request For Article
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