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The Potential Use of Nitazoxanide in the Treatment of Cutaneous Leishmaniasis: In-Vitro Assays Against Leishmania Tropica
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| Author:
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AISHAH E ALBALAWI, HAMDAN I ALMOHAMMED, AMAL KHUDAIR KHALAF, ABDULLAH D ALANAZI, ALI MOGHADDAM, MASSUMEH NIAZI, HOSSEIN MAHMOUDVAND
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| Abstract:
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Here we designed to assess the effect of nitazoxanide (NTZ) alone and along with meglumine antimoniate
(MA) against amastigote and promastigote forms of Leishmania tropica in vitro. The antileishmanial activity of
effects of NTZ alone and along with MA on L. tropica promastigote and amastigote forms have been
determined via a colorimetric MTT method and on the J774-A1 macrophage cells, respectively. Also, the effect
of NTZ on the infection rate of Leishmania to J774-A1 cells was determined. The results of the 50% inhibitory
concentrations (IC50) showed that NTZ + MA (25.8 ± 1.57 µg/ml) possess a more anti-leishmanial effect than
MA (65.3 ± 3.12 µg/ml) or MTX alone (80.2 µg/ml) on promastigotes of L. tropica. The MTX combined with
MA triggered a significant reduction (P<0.05) in the mean number of amastigotes forms in J774-A1 cells in
comparison with control group. The results also demonstrated that rate of infectivity of promastigotes was
considerably (P<0.05) decreased after it pre-incubation with NTZ. The obtained findings revealed that the
great effectiveness and a synergistic effect of NTZ alone and along with MA on the growth of L. tropica
promastigote and amastigote forms. However, further clinical studies are mandatory to examine this synergistic effect on a therapeutic scale.
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| Keyword:
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cutaneous leishmaniasis, combination therapy, promastigote, amastigote, infectivity rate
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.02.502
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| Download:
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