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Development and standardization of UVSpectrophotometric method for estimation of Azadirachtin in marketed formulation
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| Author:
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SUSHMITA I.HIREMATH, MAHESH.S.PALLED, SHAILENDRA S.SURYAWANSHI, MAHENDRA KUMAR CHOUHAN
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| Abstract:
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Objectives: The objective of present work is to develop and standardize UV-Spectrophotometric method for
estimation of Azadirachtin inmarketed formulation.
Materials and Methods: UV-Spectrophotometric method was developed using Methanol as solvent. The
developed method was standardized in terms of validation parameters such as specificity, selectivity, linear
range, precision, robustness, ruggedness and reproducibility as per ICH guidelines. Newly developed and
standardized method was successfully applied for estimation of Azadirachtin in marketed formulation.
Results: Azadirachtin exhibits ?max at 206nm and beer’s law was obeyed in the concentration range of 5-
25?g/ml. The limit of detection found to be 1.20?g/ml and limit of quantification is found to be 3.63?g/ml.
Recovery of Azadirachtin in marketed formulation was observed in the range of 100-111%. All the precision
and repeatability results were within acceptance range less than 2%. Assay of Azadirachtin was found to be
93.96%.
Conclusion: The method was found to be simple, accurate, environment friendly, reproducible and can be
used for routine estimation analysis of Azadirachtin in marketed formulation.
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| Keyword:
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Azadirachtin, Beer’s law, Methanol, UV- Spectrophotometer, Validation.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.03.063
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| Download:
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