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Isolation and identification of novel antibiotic producing bacteria Pseudomonas aeruginosa SM3 against human pathogens
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| Author:
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SRI SUSHMA MADDI, VINEELA SAI MEGAVATH, NIHARIKA BURGULA, MJM BASHA, VENKATA SAI VASHISHTA KOLLA, USHA SREE ANANDAS
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| Abstract:
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In the contemporary age, the advent and prevalence of antibiotic-resistant bacteria has become a global distress, necessitating the development of innovative broad-spectrum medicines. Antibiotic-producing bacteria were screened in soil samples from several industries and the rhizosphere in this study. For isolation of antagonistic bacteria, standard serial dilution method was used. A total of 100 morphologically distinct colonies with an evident zone of growth inhibition around them were isolated and sub-cultured as pure cultures for further research. The isolates were screened for antibiotic activity using perpendicular streaking and seed overlay methods. Only four of the 100 isolates tested positive for antibiotics, and they were examined for morphological and biochemical characteristics. Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa and Staphylococcus aureus were the four human pathogens investigated. The most promising isolate SM3 was chosen for secondary screening based on the results of primary screening. At a dose of 100µg/mL, the highest antagonistic activity against Pseudomonas and E.coli was observed with an inhibition zone of 09 and 11 mm, respectively. 16S rRNA sequencing was used to identify the isolate SM3, and the sequences were then compared using NCBI BLAST. The BLAST findings for isolate SM3 indicated 99 percent similarity with Pseudomonas aeruginosa database sequence. The sequences were submitted to GenBank and the accession number MZ323881 was obtained.
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| Keyword:
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Isolation; identification; novel antibiotic; Pseudomonas aeruginosa; human pathogens
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.03.194
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| Download:
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