*Five Years Citation in Google scholar (2016 - 2020) is. 1451*   *    IJPR IS INDEXED IN ELSEVIER EMBASE & EBSCO *       

logo

INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

A Step Towards Excellence
Published by : Advanced Scientific Research
ISSN
0975-2366
Current Issue
No Data found.
Article In Press
No Data found.
ADOBE READER

(Require Adobe Acrobat Reader to open, If you don't have Adobe Acrobat Reader)

Index Page 1
Click here to Download
IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

Click to download
 

Article Detail

Label
Label
Bioanaltical Method Development And Validation Of Levetiracetam In Rat Plasma By K3edta Using Lc-Ms/Ms.

Author: KHEDKAR AMOL NAVNATH, DR.S.SATHESH KUMAR
Abstract: Levetiracetam is a broad-spectrum antiepileptic drug with IUPAC name was (2S)-2-(2-oxopyrrolidin-1-yl) butanamide, having logP 0.64, and available dose of marketed formulation was 250 mg, 500 mg, 750 mg and half-life of 6-8 hours. Drug Stock Solution for Levetiracetam 10.000 mg/ml prepared and SS Concentrations 250000.000 ng/mL to 250.000 ng/mL for STD1 to STD9 respectively prepared and for QC Samples 200000.000 ng/mL to 250.000 ng/mL for HQC to LLOQ QC respectively, different trials were taken for optimization of chromatographic condition and mobile phase finalized was 10 mM Ammonium Acetate in Water: Acetonitrile (30:70). The stationary phase used was Gemini C18 column shows good response and good chromatography at Rt 4.3 for Levetiracetam. For extraction process P.V. 100 µl + 500 µl 2.0 % Formic Acid in Water + Conditioning (1ml Methanol+1 ml Water) + Loading + Washing (1 ml Water + 1 ml 5 % Methanol in Water) + Elution with Acetonitrile was selected on the basis of good response and good chromatography was observed at each level by using Extraction Cartridge as Strata-X 33µ (30mg/1ml). All the results were found within the acceptance criteria, and method developed and validated as per protocol successfully.
Keyword: Levetiracetam, Anti-Epileptic, validation.
DOI: https://doi.org/10.31838/ijpr/2020.12.03.523
Download: Request For Article
 
Clients

Clients

Clients

Clients

Clients
ONLINE SUBMISSION
USER LOGIN
Username
Password
Login | Register
News & Events
SCImago Journal & Country Rank

Terms and Conditions
Disclaimer
Refund Policy
Instrucations for Subscribers
Privacy Policy

Copyrights Form

0.12
2018CiteScore
 
8th percentile
Powered by  Scopus
Google Scholar

hit counters free