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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

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Published by : Advanced Scientific Research
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0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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Genetic association of ATPase Na+/K+ transporting subunit alpha 1 polymorphism with periodontitis.

Author: MEDHA RAJIV RANJAN, DR.SHIVA.P, KARTHIKEYAN MURTHYKUMAR, DHANRAJGANAPATHY
Abstract: Introduction The periodontium, which includes the gums and the tissue that surrounds the bone, is affected by the severe inflammatory illness known as periodontitis. The bacterial matrix's buildup, the tooth's singularity, bulky regions, and the gum line are the main causes of periodontitis. The function of the gene encoding ATP1A1 may be impacted by single nucleotide polymorphism. The ATP1A1 gene is responsible for forming a portion of the protein that the Na+/K+ATPase pump, which transports irons into and out of the cell and maintains the electrolyte and fluid balance, is made of. This protein is responsible for iron transport in and out of the cell. It has been reported that the ATP one-even gene is associated with the rs1060366 common gene polymorphism. Objective We hypothesized that ATP1A1 polymorphism might modify the relative risk for the development of periodontitis. On the basis of these findings, we evaluated SNP in the ATP1A1 gene in groups of non-periodontitis controls and periodontitis participants. Materials and Methods The study is a cross-sectional study involving the individuals of Chennai, Tamil Nadu. A sample of 50 human subjects was enrolled for the study, The participants were divided into a control (Group A, n=25) and periodontitis (Group B, n=25) based on the clinical examination of probing degree calculus depth, clinical attachment loss, and bleeding on probing. The periodontitis group contained 25 patients with the mean age of 39.02±8.22. The periodontitis patients were recruited based on the criteria of American Academy of Periodontology-2018 stage II and above.The control group contained 25 periodontally healthy participants with mean age of 41.34±7.49. Sample Collection and Deoxyribonucleic Acid Extraction, A volume of 2 ml of venous blood was collected from antecubital fossa and dispersed into a sterile tube containing a pinch of ethylene diaminetetraacetic acid. It was mixed thoroughly to avoid clot formation. DNA isolation was performed according to the modified Miller et al. 1998 protdegreecelciusol. Polymerase Chain Reaction and Restriction Endonuclease Digestion CCL2 (C-C motif chemokine ligand 2-rs1024611) polymorphisms were assessed by PCR amplification and restriction digestion. Results The genotypic frequencies of ATP1A1 gene polymorphism (rs1060366) among the cases and controls, with a P value of 0.0113, The genotype frequency of cases and controls, do not differ significantly. Agarose gel electrophoresis showing amplification of ATP1A1 gene spanning polymorphic site (rs1060366) run along with standard DNA ladder [ Lane M= 100 bp DNA marker]. Agarose gel electrophoretogram showing Pvull digested amplicon of ATP1A1 spanning (rs1060366) site Homozygous GG- 311bp, Heterozygous GA 94+ 217+ 311bp and Homozygous AA 94+ 217bp [ Lane M = 100 bp DNA marker ]. The Dominant model chosen for this study was found to be significant whereas the recessive model was found insignificant. The South Indian population showed the predominance of dominant alleles. Conclusion Genetic Association of the ATP1A1 gene was found with periodontitis which is helpful in early diagnosis of this disease.
Keyword: ATP1A1, Gene polymorphism, periodontitis, rs1060366, agarose gel electrophoretogram.
DOI: https://doi.org/10.31838/ijpr/2023.15.04.006
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