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Parameter Optimization for The Production of L-asparaginase by Response Surface Methodology by Beauveria bassiana SS18/41.
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| Author:
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UPLOADED BY-ADMIN, P.V. KAMALA KUMARI, G.GIRIJA SANKAR, T.PRABHAKAR
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| Abstract:
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A Central composite Design of Response Surface Methodology was used to identify the interactive effect of the four
variables dextrose, L-asparagine, pH and inoculum level on the production of L-asparaginase from Beauveria bassiana
SS18/41. Using Response Surface Methodology (RSM) it was found that a medium containing 0.43%w/v of dextrose, 1.1%
w/v of L-asparagine and pH of 7.5 of L-asparagine was optimum for production of L-asparaginase. The maximum
experimental response for L-asparaginase production was 19.64 IU/mL whereas the predicted value was 19.31 IU/mL
indicating a strong agreement between them. The model was also validated by repeating the experiments under the
optimized conditions using shake flask fermentation that resulted in L-asparaginase production of 19.88 IU/mL (predicted
response 19.90 IU/mL) that was obviously in close relation with the model prediction. The experimental values were found
to be very close to the predicted values and hence the model was successfully validated.
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| Keyword:
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Beauveria bassiana, SS18/41, submerged fermentation, L-asparaginase, CCD
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| EOI:
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| DOI:
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