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A REVIEW ON HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
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| Author:
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SHAILESH DAHIMIWAL, THORAT D.B, JAIN N.P, JADHAV V.B, PATIL P.B
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| Abstract:
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HPLC is sophisticated column chromatography technique known as high performance liquid chromatography. HPLC is a versatile tool for qualitative and quantitave estimation of various chemical compounds.
HPLC is probably the most universal type of analytical procedure; its application areas include quality control, process control, forensic analysis, environmental monitoring and clinical testing. In addition HPLC also ranks as one of the most sensitive analytical procedures and is unique in that it easily copes with multi-component mixtures. It has achieved this position as a result of the constant evolution of the equipment used in LC to provide higher and higher efficiencies at faster and faster analysis times with a constant incorporation of new highly selective column packing. A present review focused on general instrumentation, classification, principle, applications of HPLC.
UPLC system provides high speed of analyses so greater number of analyses can be performed in a shorter amount of time thereby increasing sample throughput and better assay sensitivity. Analysis of operation cost and sample throughput found UPLC cost advantageous over HPLC.
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| Keyword:
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HPLC, Chromatography, instrumentation, mobile phase
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| EOI:
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| DOI:
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