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Bioanalytical method development, validation and its application in Pharmacokinetic studies of simvastatin in the presence of piperine in rats
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| Author:
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SATISH GABHE, PRATIBHA AUTI, KAKASAHEB MAHADIK
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| Abstract:
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During our ongoing program of enhancing bioavailability of drugs by using piperine a known bioenhancer, a suitable bioanalytical method was needed. In this direction we report the development of a simple RP-HPLC bioanalytical method for determination of simvastatin in the presence of piperine. The method was carried out on RP C18 column, with mixture of 0.01% formic acid: methanol (35:65) as the mobile phase.The linearity was found to be in the range of 83.90-99.45%. The developed method was validated as per USFDA guidelines and used in the estimation of simvastatin alone and in combination with piperine on oral administration in Wistar rats. It was observed that the Cmax, AUC of simvastatin was increased by 253% and 219% respectively when it is given in combination with piperine than simvastatin alone.
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| Keyword:
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Simvastatin, Piperine, Pharmacokinetics, Bioavailability, HPLC, AUC.
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| EOI:
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| DOI:
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| Download:
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