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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

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IJPR included in UGC-Approved List of Journals - Ref. No. is SL. No. 4812 & J. No. 63703

Published by : Advanced Scientific Research
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0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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APPLICATION OF CONDENSATION TECHNIQUE FOR THE ESTIMATION OF DARUNAVIR AND EFAVIRENZ IN PURE AND TABLET FORMULATIONS

Author: R.VIJAYALAKSHMI* , D. ANJANI, M.D. DHANARAJU
Abstract: This proposed work describes two simple and fast visible spectrophotometric methods for the estimation of Darunavir with O-nitro benzaldehyde and Efavirenz with vanillin by the condensation process.Both the methods were developed on Perkin Elmer LAMBDA 25 UV –VIS spectrophotometer interfaced with UV Win lab software and 1cm quartz cells. The methods are based mainly on the reaction of the free amino group in the Darunavir with the ONB reagent and Efavirenz with the vanillin reagent undergoing condensation reaction to form colored Schiff’s bases. The methods were optimized as per standard optimization parameters.By the optimized method the pinkish brown colored chromogen for condensed DARUNAVIR was measured at 486 nm and reddish pink colored chromogen for condensed EFZ was measured at 432 nm against the reagent blank. The linearity range of Darunavir is 40-240 µg/ml and Efavirenz is 20- 100µg/ml; LOD and LOQ was found to be 2.66 and 8.87 µg/ml for Darunavir and 1.44 and 4.82 µg/ml for Efavirenz. The colorimetric methods were extensively validated as per ICH guidelines and all the parameters were within the acceptance criteria with the correlation of 0.9999 and % RSD less than 2 for both the methods. The results of the accuracy studies were nearer to 100%. The methods were proved to be more accurate, simple, precise and rapid by statistical validation as well as recovery studies and could be used for routine laboratory analysis.
Keyword: Darunavir (DNV), Efavirenz (EFZ), O-nitro Benzaldehyde (ONB), Vanillin
DOI: https://doi.org/10.31838/ijpr/2018.10.03.004
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