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RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF OLMESARTAN MEDOXOMIL AND CHLORTHALIDONE IN TABLET DOSAGE FORM
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| Author:
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VIRALI THAKKAR, R.B.PATEL, H.P.PARMAR, G.H.PATEL , P.K.PRADHAN , U.M.UPADHYAY
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| Abstract:
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Olmesartan medoxomil (OLM) is a selective AT1 subtype angiotensin II receptor antagonist and used as antihypertensive. Cholrthalidone (CLT) is one of the oldest and widely used as thiazide diuretics. It used alone or with other hypertensive drug in the management of hypertension and edema. It inhibits sodium ion transport across the renal tubular. A simple, specific, accurate and precise reverse phase High performance liquid chromatographic method was developed for the simultaneous estimation of olmesartan medoxomil and chlorthalidone ,using Acetonitrile : Methanol : Water (40:40:20 v/v/v) with pH 4.0 as the mobile phase and measuring the response at 235 nm. The analysis was performed on a Enable C18 (250 X 4.6 mm), 5.0 µ column. The Calibration curve was obtained for a range of 2-10 µg/ml and 1.25-6.25 µg/ml for OLM and CLT respectively. The mean recovery was found to be 100.13 ± 0.69 % and 99.78 ± 0.69 %. The method was validated according to the ICH guidelines and found to be in good accordance with the prescribed values. This method can be successfully employed for simultaneous quantitative analysis of Olmesartan midoxomil and Chlorthaliodone in combined dosage form.
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| Keyword:
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Chlorthalidone, Olmesartan medoxomil, RP-HPLC Method.
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| DOI:
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