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Anti-ulcerative colitis activity of Pioglitazone in rats: Role of Nitric oxide
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| Author:
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VIPIN DHOTE, BALARAMAN RAMCHANDRAN2, KAWADKAR MANISHA
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| Abstract:
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ABSTRACT
Objective: To evaluate the role of nitric oxide (NO) in pioglitazone (PIO) mediated anti-inflammatory activity in ulcerative colitis.
Material and methods: Ulcerative colitis was induced in rats by intracolonical administration of 2, 4, 6-tri-nitrobenzene sulphonic acid (50mg/rat) after 10 days of initiation of treatment except in vehicle control group.
Animals were randomly divided into five groups with six animals each. The groups received vehicle, TNBS, PIO, PIO+L-NAME (N-nitro-L-arginine methyl ester), L-NAME for 18 days respectively. PIO (25 mg/kg/day p.o) treatment for 18 days reduced mucosal damage induced by TNBS which was reflected by improvement in body weight, morphological grades of colon, reduced myeloperoxidase activity and histopathological studies. Administration of L-NAME (40 mg/kg/day, p.o) for 18 days in PIO+TNBS treated animals showed exaggerated mucosal damage as compared to PIO+TNBS treated animals.
Results: Findings indicate that L-NAME altered the protective effects of PIO in ulcerative colitis. It was also substantiated by significant decrease in NO levels of PIO+L-NAME treatment group which showed severe colonic damage. Therefore, the protection exhibited by PIO in ulcerative colitis could be mediated through NO.
Conclusion: Protective role of PIO on ulcerative colitis was markedly reversed by administration of L-NAME, which suggests pivotal role of NO in various protective mechanism exhibited by PIO in chronic inflammatory disorders.
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| Keyword:
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Pioglitazone, TNBS, Ulcerative colitis, Nitric oxide.
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| DOI:
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