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In Vitro Cytotoxicity Screening of Coumarin Compounds on Hep2 Cancer Cell line
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| Author:
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UPLOADED BY-ADMIN, RAJESH M.PATEL, N.J.PATEL
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| Abstract:
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Development of new therapeutic drugs needs assay systems to evaluate the toxicity potential of the
drugs early in the drug discovery process. In vitro cell-based cytotoxicity assay is an easy and costeffective
tool for hit ranking and lead optimization at the early stage of drug discovery. In present study,
short term cytotoxicity protocol was used to evaluate the acute toxicity potential of some coumarin derivatives
on cancer cells. we have used the coumarin derivatives namely 4-hydroxycoumarin (Compound
I), 5-chloro 4-hydroxycoumarin (Compound II) and 7-hydroxy 4-methylcoumarin(Compound III) for
screening anti cancer potential on Hep2 cells (Human laryngeal carcinoma cells; cancer cells) and Vero
cells (African green monkey kidney cells; Normal cells) by SRB, MTT and LDH assay and IC50 was determined
which was then compared for all three assay in search of the effective method of cytotoxicity
determination. The IC50 was determined by dose response curve analysis and statistical analysis, by plotting
the graph of concentration vs % growth Inhibition. The IC50 value of Compound II and III were comparably
lower than compound I on Hep2 cells in all three cytotoxicity methods proved that II & III are
good candidate may possess excellent anti cancer potentials against various malignancies.
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| Keyword:
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IC50, Cytotoxicity, Coumarin derivatives.
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| EOI:
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| DOI:
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