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Plant Tolerance and photo oxidative effect of light stress on withanolides in Withania somnifera
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| Author:
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MAMTA SHARMA, SUNIL PURI
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| Abstract:
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Withanolides, secondary metabolites present in Withania somnifera, have neuron regenerative property. The light
stress impact on morphological, physiological, biochemical parameters and on neuroactive constituent (withanolides)
were deliberated. In light treatments, one month old plants were exposed to different light regimes comprising of 8
hrs, 12 hrs, 16 hrs, 20 hrs and 24 hrs, while control plants were given 14 hrs light with light intensity of 55 µmol at
optimum temperature of 22°C. The light treatments significantly decreased stem length, root length, fresh weight
and dry weight in all the treatments except 8 hrs and 12 hrs photoperiods where there was marginal increase in stem
length due to etiolation effect. Changes in leaf area, membrane stability and relative water content was also observed
and decreased with stress. Total carbohydrates, total protein, carotenoids, tocopherol, ascorbic acid and alkaloids
decreased. The antioxidant enzymes like superoxide dismutase, catalase, peroxidase, ascorbate peroxidase,
glutathione–S-transferase, DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS (2,2’-azino-bis-3ethyl benzthiazoline-6-
sulphonic acid) enhanced due to stress treatments. Withanolides were found to be higher in long photoperiod treated
plants. W. somnifera was found to have protective mechanism against oxidative damage by maintaining higher
enzymatic and non-enzymatic antioxidants.
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| Keyword:
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Light stress, growth, oxidative stress, ROS, withanolides.
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| EOI:
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http://eoi.citefactor.org/10.11209/ijpr.10.03
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| DOI:
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https://doi.org/10.31838/ijpr/2018.10.03.061
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| Download:
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