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STERCULIA QUADRIFIDA R.BR ETHYL ACETATE FRACTION INCREASES CISPLATIN CYTOTOXICITY ON T47D BREAST CANCER CELLS
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| Author:
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ROLLANDO ROLLANDO, KESTRILIA REGA PRILIANTI
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| Abstract:
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Cisplatin is a chemotherapeutic agent used as cancer cure with side effects and resistance. Ethyl acetate fraction of
faloak bark (EAFB) are proven to have cytotoxic effect to cancer cells. This research aims to review the combination
effect of cisplatin and EAFB to the sensitivity increase on breast cancer cells, which will be confirmed through
apoptosis induction and cell cycle modulation. The cytotoxic effect was tested using MTT assay on T47D cell by using
IC50 parameter. The combination was tested by determining their combination index (CI) and cell viability. The
combination effect in apoptosis induction and cell cycle modulation were observed using flow cytometry method. The
cytotoxic test result of the combination shows CI value of below 1 at the concentration of EAFB as much as 6 µg/mL,
and cisplatin as much as 2,5 µM. The combination of EAFB and cisplatin results in phase S cell accumulation (29,98%)
on breast cancer cell T47D and was able to induct apoptosis. It is proven that ethyl acetate fraction of faloak bark can
be developed as a co-chemotherapeutic agent with cisplatin to increase the effectivity of breast cancer treatment
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| Keyword:
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Cisplatin, EAFB, cytoxicity, cell cycle, apoptosis
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| EOI:
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-
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| DOI:
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https://doi.org/10.31838/ijpr/2018.10.03.072
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| Download:
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Request For Article
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