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Efficiency of Escherichia coli in the Dissociation of Hydrocarbons and Statement their Molecular Characterization of Biodegradable
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| Author:
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BAIDAA JASSIM, ALI ABED AL-RHEEM AL-NASHI
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| Abstract:
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The study investigated the efficiency of E.coli bacteria in the degradation and consumption of hydrocarbons, isolating
23 isolates of crude oil from different environmental zones, including the (soil of the generators, the soil of the oil
change shop, the soil of the diwaniyarubber plant, the pond water). A number of variables were used to distinguish
the ability of bacterial isolates to analyze hydrocarbon compounds by their ability to grow on the medium of solid
mineral salts and the addition of crude oil. The bacterial isolates During the period of incubation for a maximum of 7
days and at a temperature of 37 ° C of consumption of crude oil as a single source of carbon and energy in addition
to its growth on the center of liquid mineral salts with crude oil by 1% The electrical conductivity and optical density
were measured. A gradual increase in the electrical conductivity and optical density of all bacterial isolates was
observed during the incubation period and E.coli isolates isolated from the water were recorded at the highest values
of 11.7mc / cm for electrical conductivity and 0.309 for optical density, the catechol 2,3 dioxygenase responsible for
the degradiationof hydrocarbons was tested and found in 6 isolates at 26.08%. The results of sequential sequence
analysis of (C23O) in E.coli showed that they were identical 100% with gene(CP019778.1). The genetic tree was also
used to show the genetic relationship between the bacterial isolates
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| Keyword:
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Phenotypic E.coli, C23O gene, Catechol enzyme 2,3 dioxygenase
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2018.10.04.106
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| Download:
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