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Molecular Characterization Based on Genetic Diversity Analysis For Fertility Restorer Genes in Rice(Oryza Sativa, L.) Using Microsatellite Markers
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| Author:
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BALQEES AL-, NIDHAL ABDUL H. AL-BDAIRI, MOHAMMED A.ALANBARI
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| Abstract:
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Rice as one of the most important agricultural crops has a putative potential for ensuring food security, The present
study was carried out with the objective to assess genetic diversity among 15 rice (Oryza sativa L.)genotypes
representative offer tility restorer genes using two specific primers of SSR markers. Results showed that the range of
molecular size (147.333 - 344.366) bp. A total of 11 alleles were amplified with a mean of 5 alleles perlocus. The
Polymorphic information content PIC values ranged from 0.5198 to 0.8006 with a mean of 0.6602.The lowest value
for main allele frequency was 0.2333 in primer RM171 while the highest value was 0.6000 for primer RM216.two
primers (RM171 and RM216) gave adistinctive genetic fingerprinting of 4 genotypes understudy Analysis of results
obtained from Neighbor-joining dendrogram revealed that, grouped all the 15 rice genotypes into two major groups:
one small cluster A and a large cluster B. The major group A comprised 6 varieties, while the cluster B comprised 9.
depending on their geographic origin, their ancestor. The results suggest microsatellite markers as a useful tool for
the estimation of genetic diversity and cultivar differentiation and present invaluable genetic information for future
breeding.
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| Keyword:
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Rice, polymorphism information contents (PIC), SSR marker,RM171.
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| EOI:
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-
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| DOI:
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https://doi.org/10.31838/ijpr/2019.11.02.015
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| Download:
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