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Cytotoxicity of Magnesium Oxide-Zinc Oxide-Titanium Nanocomposite (MgZnTiO4) on LS 174T Colorectal Adenocarcinoma Cell Line
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| Author:
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ABOTHUR MOHAMMMMED, KHALIDA K ABBAS AL-KELABY, FADHIL K FARHAN, ZAINB AL-RAMADHAN, ALI AL- MUHANA
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| Abstract:
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Many efforts had been focused on the therapeutic approach of cancer for a long time by studying cytotoxic nanoparticles'
activities. In this study, a mix of PVA 60% and PVP 35 %, with 5% reinforced bioceramic triple (ZnO, MgO, Tio2) was
applied against LS 174T colorectal adenocarcinoma cell line. This study was planned to evaluate MZT cytotoxicity by
thiazolyl blue tetrazolium bromide (MTT) cytotoxicity test on LS 174T cell line in compared with chemotherapeutic drugs
that were included Doxorubicin (DOX) and 5-flourouracil (5FU). These nanoparticles were purchased from Skyspring
Nanomaterials, Inc. USA, with 10-30 nm in diameter and 99.8%.purity. The size was further confirmed with X-ray
Diffraction (XRD), Energy Dispersive X-ray Spectroscopy (EDS) and Scanning Electron Microscopy (SEM). The results
showed that MZT nanocomposite was effectively and significantly inhibited the cell proliferation (p<0.0005) by decreasing
the viability of LS 174T cells at different concentrations involved 1, 10, 100, 1000 and 2000 µg/ml, with 5.698 µg/ml half
maximal inhibitory concentration (IC50) of. DOX and 5FU IC50 values were 27.409 µg/ml and 62.84 µg/ml, respectively. It
was concluded that MZT nanocomposite have an anticancer effect against LS 174T colorectal carcinoma and this type of
cell line was good enough for applying MTT cytotoxicity assay.
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| Keyword:
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LS 174T colorectal adenocarcinoma, Cell culture, MTT Assay, Nanoparticles.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2019.11.02.020
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| Download:
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