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Real Time – Polymerase Chain Reaction assessment of patients with bacterial meningitis in AL-Najaf province / Iraq.
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| Author:
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ENÁAM AL-JABBER, PROF. DR. BAQUR A.SULTAN, ROF. DR. HAYDER K. HASSOUN
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| Abstract:
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This study was conducted on 200 children and adult of both sexes who attended Al- Zahra Maternity and Children
Teaching Hospital &Middle Euphrates Neuroscience Center in Al-Najaf province from July 2016 to July 2017 because
they complaining of some symptoms that may related to meninges infection. The mean ages of suspected patients was
13.09 years for (109) males ,and 11.28 years for (91)females.CSF - NHS Meningitidis Real-Time PCR kit is a Real-Time
test for the detection and differentiation of Neisseria meningitidis, Haemophilus influenzae and Streptococcus
pneumoniae in clinical samples using the TaqMan system. Out of 200 CSF sample only 42 were positive for S.
pneumoniae (7 male – 6 female ), 21 N. meningitidis (10 male -11 female ) and for 8 H. influenzae (3 male-5 female). S.
pneumoniae and H. influenzae was the predominant microorganism among children from ?1 to 9 years old, while N.
meningitides was the predominant causing bacterial meningitis in ?10 years. The developed real time PCR is suitable
for identification and quantification of H. influenzae type b which will be helpful in diagnosis, and treatment in future.
Attention must be paid to public health. Published audio and video media on how to prevent meningitis.National
Vaccination Plan for bacterial and viral meningitis.Give priority to find fast laboratory diagnosis before therapy.
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| Keyword:
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CSF, Bacterial meningitis, S. pneumoniae, H. influenzae, N. meningitidis, PCR.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2019.11.02.023
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| Download:
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