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Estimation and Extraction of poly-ß- hydroxybutyrate in Bacillus cereus M13 with different conditions.
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| Author:
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NOORA AMIR, AL-WATIFIWEJDAN, RIDHA TAJ-AL-DEEN, ANWAR KADHM AL-SAFFAR
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| Abstract:
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quantity of synthetic plastic refuse incrementeach year addition to take long period to degradation, beside that
produce toxic materials affecting in environment. This problem can solve by use bioplastic, the isolates from soil
polluted by hydrocarbon materials in different position in Babylon/ Iraq. Bacillus cereus strainM13 was the isolate
produce poly-ß-hydroxybutyrate in high yields. Tow fluorescent stain were used to screened about PHB production,
Nile blue A and Nile red. the maximum PHB yields was appear when use glucose at 5% concentration as a carbon
source, and yeast extract at concentration 0.5g/100ml as a nitrogen source. Regarding agitation speed, inoculum size,
temperature, incubation time and pH, the optimum conditions of PHB yields were 150 rpm, 10%,37°C, 72 h and 8,
respectively. Bacillus cereus strainM13 was capable of accumulating PHB from Glucose, Sucrose, Coffee waste ,
Rice bran, Wheat bran, Molasses and Potato waste that used as ananagrammatic carbon sources for the PHB yields.
Production of PHB was elevated from 4.3% to 40% in HM medium.
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| Keyword:
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poly-ß-hydroxybutyrate, triosephosphateisomerase, optimization, Bacillus cereusM13.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2019.11.02.028
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| Download:
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