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Safety Studies of Bisehri Booti (Aerva lanata Linn.)–A Frequently used Unani Medicinal plant as medicament
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| Author:
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NAJMUDDIN SIDDIQUI, ASMA ABID, MOHAMMAD ZAKIR, MASROOR ALI QURESHI, AMIR AZAM
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| Abstract:
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WHO has made it mandatory to do safety studies of herbal medicines and food items in order to prevent the
possible toxicity caused by the contamination of toxic materials found in the soils. The aim of the present study was
to determine the concentration of aflatoxin, heavy metal, pesticidal residue, microbial load in the market sample of
the Bisehri Booti (Aerva lanata L.) and cytotoxicity study was also conducted. Load Determination Test for each
contaminant was done to determine the load of specific contaminants in test drug. The total Bacterial and yeast
Count was determined using plate count method for bacteria, fungi and specific pathogens. LCMS-MS was used to find
presence of aflatoxins e.g. Aflatoxins B1, aflatoxins G1, aflatoxin B2 and aflatoxin G2. GCMS-MS was used to
determine the Specific pesticide residues such as organo-chlorine, organo-phosphorous and Pyrethroids compounds.
Heavy metals including Arsenic, Mercury, Cadmium and Lead were determined by using AAS technique. Cytotoxicity
study of the normal cells eg. HEK293 was evaluated with the help of MTT assay. Bisehri Booti (Aerva lanata L.) was
found to possess aflatoxin, pesticidal residue and microbial but well below the permissible limits, while the heavy
metals were quietly absent and more than 75% cells were viable even at 250 µg/ml concentration of extracts. The
results concluded that market sample is safe and may be used as a therapeutic agent.
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| Keyword:
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Cytotoxicity, Safety Profile, Bisehri Booti, WHO guidelines, Heavy metals
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2019.11.02.049
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| Download:
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