|
Reduction of Cisplatin Toxicity in Vero Cell Lines by Pedalium murex Linn. Leaf Extract
|
|
| Author:
|
I ALAM, V GOPAL, C VASANTHI, P K MANNA
|
| Abstract:
|
Nephrotoxicity is the damage/destruction of kidney function by exogenous or endogenous toxicants. Drug induced
nephrotoxicity is a common problem in many vital treatments. Cisplatin is highly effective to broad spectrum of
malignancies, its adverse effect of nephrotoxicity limits its use. Pedalium murex is an annual herb with wide range of
phytoconstituents and pharmacological activity. In this research work it was aimed to reveal the nephroprotective
property of leaf of Pedalium murex. Different extracts of P. murex leaf was subjected to DPPH, ABTS & NO
antioxidant assay and in-vitro nephroprotective activity on vero cell lines. Cytotoxicity was induced by Cisplatin and
the cell viability was studied by MTT assay. Among the different extracts, aqueous(PLW) and ethanolic(PLE) extracts
exhibited good DPPH scavenging activity than chloroform and n-hexane extracts with IC50 value of 21.14±2.65 µg/ml
and 16.18±0.79 µg/ml respectively. Whereas for gallic acid IC50 was 3.19±2.33 µg/ml. In ABTS and NO assay PLE
showed significantly more scavenging property than PLW. Total phenolic content in PLE (61.18±2.36 mg GAE/g) was
more than PLW (36.80±1.86 mg GAE/g). In-vitro nephroprotectie analysis revealed that PLE possess more
nephroprotective with percentage cell viability of 96.93±1.04% than PLW with 86.65±1.87% at 200µg/ml
concentration. Standard drug Quercetin showed 90.68±0.11% cell viability at 100µg/ml. The results indicates that
ethanolic extract of Pedalium murex effectively reduces cisplatin toxicity in cultured vero cells. The scavenging of free
radicals may be the important role in the protection, as reactive oxygen species (ROS) generation and oxidative
stress is the major reason for cisplatin nephrotoxicity.
|
| Keyword:
|
Nephroprotective, Cisplatin, Vero cells, Nephrotoxicity, Pedalium murex, cytotoxicity
|
| EOI:
|
-
|
| DOI:
|
https://doi.org/10.31838/ijpr/2019.11.02.058
|
| Download:
|
Request For Article
|
|
|